Hello, I am dealing with a Zn-protein and have data to fit... Dealing with the old question of background correction, I have several DIFFERENT recipes from SK and others. Especially the alignment of theory and experiment. Is there anyone willing to give me a definitive protocol? In particular, SK writes that one should "read the data into Artemis": now, Artemis does not read the crude data and I have to go through Athena, as far as I understand. But when I read the athena.prj into Artemis, will the imported data be corrected already using the default values for the spline as provided in Athena? If so, there is already an initial bias, I think. Help is welcome Stefano -- ____________________________________________ Stefano Ciurli Professor of Chemistry Laboratory of Bioinorganic Chemistry Department of Agro-Environmental Science and Technology University of Bologna Viale Giuseppe Fanin, 40 I-40127 Bologna Italy Phone: +39-051-209-6204 Fax: +39-051-209-6203 "Fatti non foste a viver come bruti, ma per seguir virtute e canoscenza" Dante Alighieri - Inferno - Canto XXVI "Ihr seid bestimmt, nicht Tieren gleich zu leben, Nein, Tugend zu erringen und Erkenntnis" "Ye were not form'd to live the life of brutes, But virtue to pursue and knowledge high"