Juan, There are 2 ways to apply the self-absorption correction in Athena. The first way is to pick thicknesses starting with a couple microns and going thicker and thicker until the correction has no effect. I like this method because it gives an idea of the effects that thickness and concentration can have on the fluorescence. The other way is to simply use a thickness that will be in the infinite thickness domain to begin with. Either way, you get the information needed. To do the self-absorption correction in Athena go to Data then self-absorption, pick your algorithm (I suggest reading the Athena document section to see which you should use), plug in the formula for the sample and then either plot the information depth or plot data and correction. Rich Juan Antonio Maciá Agulló wrote:
Ok, how can I apply an "infinite" sample correction? The other samples are measured also in fluorescence mode, then is it better to measure Ptfoil in the same conditions (fluorescence)?
Thanks a lot
Best regards, JA
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