On Tue, 5 Dec 2006, Juan Antonio Maciá Agulló wrote:
Ok, how can I apply an "infinite" sample correction? The other samples are measured also in fluorescence mode, then is it better to measure Ptfoil in the same conditions (fluorescence)?
The question about applying the correction has been answered. As to your question about the standard being measured the same way as the samples. yes, it is important to have standards be the same but not if you are compromising the data quality. If the sample is mad efor transmission it is not necessarily the right sample to measure in fluorescence. Perhaps a thin film of Pt would be a better fluorescence sample. Something with signficantly less than an absorption length of thickness. In my experience, mixing modes (transmission and fluorescence) is OK if you are careful about drawing conclusions. For example, a Pt foil is a geed standard because it permits you to see if you can get a consistent amplitude reduction factor which you can then use for your nanoparticles. For this purpose transmission is fine. If you want a Pt standard that is like your samples, then Pt black would be better but you know that it will have a reduced SO2 as well because it is in nanoparticle form. Cheers, Carlo
Thanks a lot
Best regards, JA
-- Carlo U. Segre -- Professor of Physics Associate Dean for Special Projects, Graduate College Illinois Institute of Technology Voice: 312.567.3498 Fax: 312.567.3494 segre@iit.edu http://www.iit.edu/~segre segre@debian.org